USE OF 2-DIMENSIONAL THIN-LAYER CHROMATOGRAPHY FOR THE COMPONENTS STUDY OF POLY(ADENOSINE DIPHOSPHATE RIBOSE)

被引：40

作者：

KEITH, G

DESGRES, J

DEMURCIA, G

机构：

[1] UNIV BOURGOGNE,BIOCHIM MED LAB,F-21034 DIJON,FRANCE

[2] UNIV STRASBOURG 1,F-67084 STRASBOURG,FRANCE

来源：

ANALYTICAL BIOCHEMISTRY | 1990年 / 191卷 / 02期

关键词：

D O I：

10.1016/0003-2697(90)90224-W

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Two-dimensional thin-layer chromatography on cellulose plates has been used for separating and quantifying the three adenosine derivatives: AMP, phosphoribosyl AMP (PRAMP), and (PR)2AMP obtained by venom phosphodiesterase digestion of poly (ADP-ribose). In vitro synthesized polymer, up to 300 derivatives in length were studied. Some parameters of the complexity of poly (ADP-ribose) could be deduced from our results: (i) The first branching point appears in fragments of approximately 21 derivatives in length, (ii) The branching points are located at regular distances of approximately 41 derivatives from each other. © 1990.

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页码：309 / 313

页数：5

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