METHOD FOR CONSTRUCTION OF SPECIALIZED TRANSDUCING PHAGE-RHO-11 OF BACILLUS-SUBTILIS

被引：81

作者：

KAWAMURA, F

SAITO, H

IKEDA, Y

机构：

[1] Institute of Applied Microbiology, The University of Tokyo, Bunkyo-ku, Tokyo

来源：

GENE | 1979年 / 5卷 / 02期

关键词：

in vitro recombination; phage vector; Recombinant DNA;

D O I：

10.1016/0378-1119(79)90095-7

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

DNA from a temperate phage ρ{variant}11 and chromosomal DNA of Bacillus subtilis 168 were digested with endonuclease EcoRI and then ligated with T4 polynucleotide ligase. The ligated DNA fragments were used to transform a lysogenic strain, B. subtilis spoA12 lys21 hisA1 leuA8 ρ{variant}11, and Lys+, His+ or Leu+ transformants were selected. The cells of each type were then mixed, grown and treated with mitomycin C; the induced phages were tested for abilities abilities to form plaques and to transduce the auxotrophic marker. Various types of plaque-forming or defective phages which transduce hisA or lys marker at considerably high frequencies were thus obtained. © 1979.

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页码：87 / 91

页数：5

相关论文

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