COMPLEMENTATION BY SR PROTEINS OF PRE-MESSENGER-RNA SPLICING REACTIONS DEPLETED OF U1 SNRNP

被引：150

作者：

CRISPINO, JD

BLENCOWE, BJ

SHARP, PA

机构：

[1] MIT,CTR CANC RES,CAMBRIDGE,MA 02139

[2] MIT,DEPT BIOL,CAMBRIDGE,MA 02139

来源：

SCIENCE | 1994年 / 265卷 / 5180期

关键词：

D O I：

10.1126/science.8091213

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Individual small nuclear ribonucleoproteins (snRNPs) U1, U2, and U4/U6 were removed from nuclear extracts of HeLa cells by antisense affinity depletion. Addition of a highly purified preparation of SR proteins fully restored splicing activity in reactions depleted of U1 snRNP but did not reconstitute splicing in reactions depleted of the other snRNPs. Affinity selection experiments revealed that spliceosomes lacking U1 snRNA formed in the U1 snRNP-depleted reactions reconstituted with SR proteins. Thus, high concentrations of SR proteins facilitate the assembly of precursor messenger RNA (pre-mRNA) into a spliceosome in the absence of interactions with U1 snRNP.

引用

页码：1866 / 1869

页数：4

共 30 条

[1] TARGETED SNRNP DEPLETION REVEALS AN ADDITIONAL ROLE FOR MAMMALIAN U1 SNRNP IN SPLICEOSOME ASSEMBLY [J].