VIDEO CAMERA CALIBRATION FOR OPTICAL DENSITOMETRY

被引：17

作者：

BALDOCK, RA

POOLE, I

机构：

[1] MRC Human Genetics Unit, Edinburgh, EH4 2XU, Crewe Road

来源：

JOURNAL OF MICROSCOPY-OXFORD | 1993年 / 172卷

基金：

英国医学研究理事会;

关键词：

OPTICAL DENSITY; CAMERA CALIBRATION; SHADING CORRECTION; MICROSCOPIC IMAGES;

D O I：

10.1111/j.1365-2818.1993.tb03392.x

中图分类号：

TH742 [显微镜];

学科分类号：

摘要：

An efficient technique for calibrating video cameras to record optical density (OD) from microscopic images is described. The method corrects for variation over the field of the brightfield and darkfield intensities, does not assume a linear response of the camera to the incident intensity and requires a single calibration filter.

引用

页码：49 / 54

页数：6

共 8 条

[1] A CHARGE COUPLED DEVICE-BASED IMAGE CYTOPHOTOMETRY SYSTEM FOR QUANTITATIVE HISTOCHEMISTRY AND CYTO-CHEMISTRY [J].