RAPID-DETERMINATION OF CHAIN-LENGTH PATTERN IN POLY(ADP-RIBOSE) SAMPLES

被引：25

作者：

ADAMIETZ, P

BREDEHORST, R

HILZ, H

机构：

[1] Institut für Physiologische Chemie, Universität Hamburg

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1978年 / 81卷 / 04期

关键词：

D O I：

10.1016/0006-291X(78)91288-3

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

(3H)Poly(ADP-ribose) synthesized from nuclei by incubation with (3H)NAD was released from protein by alkaline treatment and electrophoresed in dodecyl sulfate gels. Individual polymers up to at least 33 units were completely separated according to their chain length. Size distribution was visualized by fluorography of the gels, and quantified by radioactivity determination of sliced gels. The method could be applied to crude nuclear extracts. It showed that nuclei of Ehrlich ascites [mouse] tumor cells produced a poly(ADP-ribose) pattern distinctly different from that of rat liver nuclei.

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页码：1377 / 1383

页数：7

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