A RECOMBINANT FOWLPOX VIRUS EXPRESSING THE HEMAGGLUTININ-NEURAMINIDASE GENE OF NEWCASTLE-DISEASE VIRUS (NDV) PROTECTS CHICKENS AGAINST CHALLENGE BY NDV

被引：91

作者：

BOURSNELL, MEG ^{[1
]}

GREEN, PF ^{[1
]}

SAMSON, ACR ^{[1
]}

CAMPBELL, JIA ^{[1
]}

DEUTER, A ^{[1
]}

PETERS, RW ^{[1
]}

MILLAR, NS ^{[1
]}

EMMERSON, PT ^{[1
]}

BINNS, MM ^{[1
]}

机构：

[1] UNIV NEWCASTLE UPON TYNE, SCH MED, DEPT BIOCHEM & GENET, NEWCASTLE UPON TYNE NE1 7RU, TYNE & WEAR, ENGLAND

来源：

VIROLOGY | 1990年 / 178卷 / 01期

关键词：

D O I：

10.1016/0042-6822(90)90408-J

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

The hemagglutinin-neuraminidase (HN) gene from the Beaudette C strain of Newcastle disease virus (NDV) has been expressed in a recombinant fowlpox virus vector. The HN gene, under the control of the vaccinia p7.5 promoter, was inserted into a nonessential gene in the terminal inverted repeats of fowlpox virus. Expression was demonstrated in tissue culture, a protein of the correct size for fully glycosylated HN protein being recognized by an HN-specific monoclonal antibody on Western blots. When the recombinant fowlpox virus was inoculated into chickens by intravenous or wing-web routes, antibody which recognizes HN from purified NDV virions wasinoculated. Protective immunity to NDV was generated in the chickens; at the highest dose of vaccine 100% of the chickens tested were protected against challenge with a virulent strain of NDV. © 1990.

引用

页码：297 / 300

页数：4

共 23 条

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