A RAPID IMMUNOSTAINING METHOD FOR FROZEN-SECTIONS

被引：52

作者：

CHILOSI, M ^{[1
]}

LESTANI, M ^{[1
]}

PEDRON, S ^{[1
]}

MONTAGNA, L ^{[1
]}

BENEDETTI, A ^{[1
]}

PIZZOLO, G ^{[1
]}

MENESTRINA, F ^{[1
]}

机构：

[1] UNIV VERONA,DEPT HEMATOL,I-37100 VERONA,ITALY

来源：

BIOTECHNIC & HISTOCHEMISTRY | 1994年 / 69卷 / 04期

关键词：

IMMUNOHISTOCHEMISTRY; FROZEN SECTIONS; MICROWAVE; EPOS;

D O I：

10.3109/10520299409106292

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

A simple and rapid one-step method for demonstrating immunohistochemical markers (leukocyte common antigen, cytokeratin, etc.) is described, which can help define the nature of poorly differentiated neoplasms for diagnosis using frozen section. Microwave irradiation was used to speed immunohistochemical analysis using ''Enhanced Polymer One-step Staining'' (EPOS) reagents on cryostat sections from a variety of pathologic samples. Reproducible results were obtained using EPOS reagents for leukocyte common antigen and cytokeratin. The overall procedure takes less than 10 min and can be completed during surgery.

引用

收藏

页码：235 / 239

页数：5

相关论文

共 13 条

[1] THE USE OF ANTIKERATIN ANTISERUM AS A DIAGNOSTIC TOOL - THYMOMA VERSUS LYMPHOMA [J].

BATTIFORA, H ;

SUN, TT ;

BAHU, RM ;

RAO, S .

HUMAN PATHOLOGY, 1980, 11 (06) :635-641

[2] ACCELERATED IMMUNOGOLD SILVER AND IMMUNOPEROXIDASE STAINING OF PARAFFIN SECTIONS WITH THE USE OF MICROWAVE IRRADIATION - FACTORS INFLUENCING RESULTS [J].

BOON, ME ;

KOK, LP ;

MOORLAG, HE ;

SUURMEIJER, AJH .

AMERICAN JOURNAL OF CLINICAL PATHOLOGY, 1989, 91 (02) :137-143

[3]

CHILOSI M, 1981, AM J CLIN PATHOL, V75, P638

[4] MULTIMARKER IMMUNOHISTOCHEMICAL STAINING OF CALGRANULINS, CHLOROACETATE ESTERASE, AND S100 FOR SIMULTANEOUS DEMONSTRATION OF INFLAMMATORY CELLS ON PARAFFIN SECTIONS [J].

CHILOSI, M ;

MOMBELLO, A ;

MONTAGNA, L ;

BENEDETTI, A ;

LESTANI, M ;

SEMENZATO, G ;

MENESTRINA, F .

JOURNAL OF HISTOCHEMISTRY & CYTOCHEMISTRY, 1990, 38 (11) :1669-1675

[5] A 30-S PAS STAIN FOR FROZEN-SECTIONS [J].

DWORAK, O ;

WITTEKIND, C .

AMERICAN JOURNAL OF SURGICAL PATHOLOGY, 1992, 16 (01) :87-88

[6]

GATTER KC, 1985, LANCET, V1, P1302

[7] MAJOR IMPROVEMENT IN MICROSCOPIC-IMAGE QUALITY OF CRYOSTAT SECTIONS - COMBINING FREEZING AND MICROWAVE-STIMULATED FIXATION [J].

KOK, LP ;

BOON, ME ;

SUURMEIJER, AJH .

AMERICAN JOURNAL OF CLINICAL PATHOLOGY, 1987, 88 (05) :620-623

[8] RAPID IMMUNOPEROXIDASE STAINING OF LYMPHOCYTE ANTIGENS USING MICROWAVE IRRADIATION [J].

LEONG, ASY ;

MILIOS, J .

JOURNAL OF PATHOLOGY, 1986, 148 (02) :183-187

[9] A RAPID IMMUNOSTAINING METHOD UTILIZING PREFORMED ANTIBODY-AVIDIN-BIOTIN-PEROXIDASE COMPLEXES [J].

LLOYD, RV ;

SCHMIDT, K ;

BLAIVAS, L ;

MCCOY, JP ;

WILSON, BS .

AMERICAN JOURNAL OF CLINICAL PATHOLOGY, 1985, 83 (05) :636-639

[10]

PIZZOLO G, 1980, CANCER, V46, P2640, DOI 10.1002/1097-0142(19801215)46:12<2640::AID-CNCR2820461218>3.0.CO