FUNCTIONAL DIFFERENCE BETWEEN SERCA2A AND SERCA2B CA2+ PUMPS AND THEIR MODULATION BY PHOSPHOLAMBAN

被引：126

作者：

VERBOOMEN, H

WUYTACK, F

DESMEDT, H

HIMPENS, B

CASTEELS, R

机构：

[1] Laboratorium voor Fysiologie, Katholieke Universiteit Leuven, Campus Gasthuisberg, B-3000 Leuven

来源：

BIOCHEMICAL JOURNAL | 1992年 / 286卷

关键词：

D O I：

10.1042/bj2860591

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

COS 1 cells were transfected with full-length pig stomach sarcoplasmic/endoplasmic reticulum Ca2+ pump (SERCA)2a or SERCA2b cDNA. Ca2+ uptake by microsomes from transfected cells revealed that the Ca2+ affinity of the SERCA2b Ca2+ pump (K0.5 0.17 +/- 0.01-mu-M) was higher than that of the SERCA2a Ca2+ pump (K0.5 0.31 +/- 0.02-mu-m). Thapsigargin-sensitivity was found to be identical for the two isoforms. The Ca2+ affinity of both the SERCA2a and SERCA2b Ca2+ pumps was decreased by a factor of two when they were co-expressed with phospholamban.

引用

页码：591 / 595

页数：5

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