STABLE NUCLEOSOME POSITIONING AND COMPLETE REPRESSION BY THE YEAST ALPHA-2 REPRESSOR ARE DISRUPTED BY AMINO-TERMINAL MUTATIONS IN HISTONE-H4

被引：147

作者：

ROTH, SY ^{[1
]}

SHIMIZU, M ^{[1
]}

JOHNSON, L ^{[1
]}

GRUNSTEIN, M ^{[1
]}

SIMPSON, RT ^{[1
]}

机构：

[1] UNIV CALIF LOS ANGELES, DEPT BIOL, INST MOLEC BIOL, LOS ANGELES, CA 90024 USA

来源：

GENES & DEVELOPMENT | 1992年 / 6卷 / 03期

关键词：

ALPHA-2; REPRESSION; NUCLEOSOME; HISTONE-H4; TRANSCRIPTION; CHROMATIN;

D O I：

10.1101/gad.6.3.411

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

Nucleosomes are positioned in the presence of the yeast repressor-alpha-2 in minichromosomes containing the alpha-2 operator and on the promoters of a-cell-specific genes regulated by alpha-2. To investigate the possibility that alpha-2 directs nucleosome position through an interaction with a component of the core particle, we analyzed chromatin structures adjacent to the operator in alpha-cells containing mutations in the amino-terminal region of histone H4. Deletion or point mutation of specific amino acids in histone H4 altered the location and/or stability of nucleosomes adjacent to the alpha-2 operator. These changes in chromatin structure were accompanied by partial derepression of a beta-galactosidase reporter construct under alpha-2 control, even though alpha-2 remained bound to its operator sequence. Our data suggest that complete repression by alpha-2 requires stable positioning of nucleosomes in promoter regions and this positioning involves the conserved amino-terminal region of histone H4.

引用

页码：411 / 425

页数：15

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