CLONED AND EXPRESSED NITRIC-OXIDE SYNTHASE STRUCTURALLY RESEMBLES CYTOCHROME-P-450 REDUCTASE

被引：2301

作者：

BREDT, DS

HWANG, PM

GLATT, CE

LOWENSTEIN, C

REED, RR

SNYDER, SH

机构：

[1] JOHNS HOPKINS MED INST,DEPT NEUROSCI,725 N WOLFE ST,BALTIMORE,MD 21205

[2] JOHNS HOPKINS MED INST,DEPT PHARMACOL & MOLEC SCI,BALTIMORE,MD 21205

[3] JOHNS HOPKINS MED INST,DEPT PSYCHIAT & BEHAV SCI,BALTIMORE,MD 21205

[4] JOHNS HOPKINS UNIV,SCH MED,HOWARD HUGHES MED INST,DEPT MOLEC BIOL & GENET,BALTIMORE,MD 21205

来源：

NATURE | 1991年 / 351卷 / 6329期

关键词：

D O I：

10.1038/351714a0

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Nitric oxide is a messenger molecule, mediating the effect of endothelium-derived relaxing factor in blood vessels and the cytotoxic actions of macrophages, and playing a part in neuronal communication in the brain. Cloning of a complementary DNA for brain nitric oxide synthase reveals recognition sites for NADPH, FAD, flavin mononucleotide and calmodulin as well as phosphorylation sites, indicating that the synthase is regulated by many different factors. The only known mammalian enzyme with close homology is cytochrome P-450 reductase.

引用

页码：714 / 718

页数：5

共 48 条

[1] NITRIC-OXIDE ACTIVATES GUANYLATE CYCLASE AND INCREASES GUANOSINE 3'-5'-CYCLIC MONOPHOSPHATE LEVELS IN VARIOUS TISSUE PREPARATIONS [J].