PRIMARY STRUCTURE OF A CHITINASE-ENCODING GENE (CHI1) FROM THE FILAMENTOUS FUNGUS APHANOCLADIUM-ALBUM - SIMILARITY TO BACTERIAL CHITINASES

被引：44

作者：

BLAISEAU, PL ^{[1
]}

LAFAY, JF ^{[1
]}

机构：

[1] UNIV PARIS 11,INST GENET & MICROBIOL,BAT 400,F-91405 ORSAY,FRANCE

来源：

GENE | 1992年 / 120卷 / 02期

关键词：

HYPERPARASITIC FUNGUS; EXTRACELLULAR ENZYME; ACTIVE SITE; LEADER SEQUENCE; SEQUENCE COMPARISONS;

D O I：

10.1016/0378-1119(92)90099-B

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

Chitinase 1 (Chi1) is the major extracellular chitinase from the hyperparasitic fungus, Aphanocladium album. We determined the complete sequence of the chromosomal and cDNA copies of the structural gene (chi1) coding for Chi 1. The coding region is interrupted by three short introns (55, 53 and 49 bp long). Chi1 is 423 aa long and begins with a stretch of 34 aa not found in the mature protein. The Chi1 sequence presents overall similarities with bacterial chitinases from Serratia marcescens and Bacillus circulans. Compared with other chitinases, A. album Chi1 has only two short similarity regions (12 and 8 aa long), which are also found in bacterial, yeast and some plant chitinases.

引用

页码：243 / 248

页数：6

共 38 条

[1] CELL WALL CHEMISTRY MORPHOGENESIS AND TAXONOMY OF FUNGI [J].

BARTNICK.S .

ANNUAL REVIEW OF MICROBIOLOGY, 1968, 22 :87-+

[2] CLONING AND EXPRESSION OF A CHITINASE GENE FROM THE HYPERPARASITIC FUNGUS APHANOCLADIUM-ALBUM [J].

BLAISEAU, PL ;

KUNZ, C ;

GRISON, R ;

BERTHEAU, Y ;

BRYGOO, Y .

CURRENT GENETICS, 1992, 21 (01) :61-66

[3] CHITINASE IN BEAN-LEAVES - INDUCTION BY ETHYLENE, PURIFICATION, PROPERTIES, AND POSSIBLE FUNCTION [J].

BOLLER, T ;

GEHRI, A ;

MAUCH, F ;

VOGELI, U .

PLANTA, 1983, 157 (01) :22-31

[4] MULTIPLE SEQUENCE ALIGNMENT WITH HIERARCHICAL-CLUSTERING [J].

CORPET, F .

NUCLEIC ACIDS RESEARCH, 1988, 16 (22) :10881-10890

[5]

Dayhoff MO, 1978, ATL PROTEIN SEQ STRU, V5, P345

[6]

DELTOUR L, 1992, Methods in Molecular and Cellular Biology, V3, P35

[7]

FOUCHE PB, 1978, J BIOL CHEM, V253, P6787

[8] DOMAINS IN MICROBIAL BETA-1,4-GLYCANASES - SEQUENCE CONSERVATION, FUNCTION, AND ENZYME FAMILIES [J].

GILKES, NR ;

HENRISSAT, B ;

KILBURN, DG ;

MILLER, RC ;

WARREN, RAJ .

MICROBIOLOGICAL REVIEWS, 1991, 55 (02) :303-315

[9]

Gurr SJ, 1987, GENE STRUCTURE EUKAR, P93

[10] NUCLEOTIDE-SEQUENCE OF THE CHITINASE-B GENE OF SERRATIA-MARCESCENS QMB1466 [J].

HARPSTER, MH ;

DUNSMUIR, P .

NUCLEIC ACIDS RESEARCH, 1989, 17 (13) :5395-5395

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