2-AMINOPURINE INHIBITS THE DOUBLE-STRANDED RNA-DEPENDENT PROTEIN-KINASE BOTH IN-VITRO AND IN-VIVO

被引:109
作者
HU, YH [1 ]
CONWAY, TW [1 ]
机构
[1] UNIV IOWA,DEPT BIOCHEM,IOWA CITY,IA 52242
来源
JOURNAL OF INTERFERON RESEARCH | 1993年 / 13卷 / 05期
关键词
D O I
10.1089/jir.1993.13.323
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The autophosphorylation of interferon (IFN)-induced double-stranded RNA-dependent p68 protein kinase (PKR) and phosphorylation of the alpha-subunit of the translation initiation factor eIF-2 were inhibited by 10 mM 2-aminopurine in vitro. High concentrations of ATP overcame the inhibition. Kinetic studies indicated that 2-aminopurine is a competitive inhibitor with respect to ATP, suggesting that these two molecules bind the same site on the kinase. Treatment of HeLa cells with poly(l):poly(C) stimulated PKR autophosphorylation in vivo. The stimulated activity was inhibited by 10 mM 2-aminopurine to approximately the same extent as the in vitro inhibition.
引用
收藏
页码:323 / 328
页数:6
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