QUANTITATION OF ANGIOGENESIS AND ANTIANGIOGENESIS IN THE CHICK-EMBRYO CHORIOALLANTOIC MEMBRANE

被引：196

作者：

NGUYEN, M

SHING, Y

FOLKMAN, J

机构：

[1] BRIGHAM & WOMENS HOSP,DEPT SURG,BOSTON,MA 02115

[2] HARVARD UNIV,SCH MED,DEPT SURG ANAT & CELLULAR BIOL,BOSTON,MA 02115

[3] HARVARD UNIV,SCH MED,DEPT BIOL CHEM,BOSTON,MA 02115

来源：

MICROVASCULAR RESEARCH | 1994年 / 47卷 / 01期

关键词：

D O I：

10.1006/mvre.1994.1003

中图分类号：

R6 [外科学];

学科分类号：

1002 ; 100210 ;

摘要：

A novel method for quantitating angiogenesis and its inhibition has been developed for the chick embryo. The method is based on the vertical growth of new capillary blood vessels into a collagen gel through two parallel nylon meshes which align the capillaries for counting. Angiogenesis is induced by basic fibroblast growth factor contained within the gel and slowly released by aluminum sucrose octasulfate (sucralfate) or by tumor cells implanted on the gel. The potency of four different angiogenesis inhibitors was compared at concentrations of 0.6 to 600 nmole. This technique may facilitate the discovery and development of angiogenesis inhibitors for clinical application. © 1994 Academic Press, Inc.

引用

页码：31 / 40

页数：10

共 31 条

[1] SIMPLE PROCEDURE FOR LONG-TERM CULTIVATION OF CHICKEN EMBRYOS [J].