AKT2, A PUTATIVE ONCOGENE ENCODING A MEMBER OF A SUBFAMILY OF PROTEIN-SERINE THREONINE KINASES, IS AMPLIFIED IN HUMAN OVARIAN CARCINOMAS

被引：639

作者：

CHENG, JQ ^{[1
]}

GODWIN, AK ^{[1
]}

BELLACOSA, A ^{[1
]}

TAGUCHI, T ^{[1
]}

FRANKE, TF ^{[1
]}

HAMILTON, TC ^{[1
]}

TSICHLIS, PN ^{[1
]}

TESTA, JR ^{[1
]}

机构：

[1] FOX CHASE CANC CTR,DEPT MED ONCOL,PHILADELPHIA,PA 19111

来源：

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA | 1992年 / 89卷 / 19期

关键词：

SRC HOMOLOGY 2-LIKE DOMAIN; GENE AMPLIFICATION; CHROMOSOME ALTERATIONS;

D O I：

10.1073/pnas.89.19.9267

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

We isolated cDNA clones containing the entire coding region of the putative oncogene AKT2. Sequence analysis and in vitro translation demonstrated that AKT2 encodes a 56-kDa protein with homology to serine/threonine kinases; moreover, this protein contains a Src homology 2-like domain. AKT2 was shown to be amplified and overexpressed in 2 of 8 ovarian carcinoma cell lines and 2 of 15 primary ovarian tumors. AKT2 was mapped to chromosome region 19q13.1-q13.2 by fluorescence in situ hybridization. In the two ovarian carcinoma cell lines exhibiting amplification of AKT2, the amplified sequences were localized within homogeneously staining regions. We conclude that AKT2 belongs to a distinct subfamily of protein-serine/threonine kinases containing Src homology 2-like domains and that alterations of AKT2 may contribute to the pathogenesis of ovarian carcinomas.

引用

页码：9267 / 9271

页数：5

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