EXPRESSION OF CYSTEINE PROTEINASES BY METACYCLIC PROMASTIGOTES OF LEISHMANIA-MEXICANA

被引：19

作者：

BATES, PA ^{[1
]}

ROBERTSON, CD ^{[1
]}

COOMBS, GH ^{[1
]}

机构：

[1] UNIV GLASGOW,DEPT ZOOL,BIOCHEM PARASITOL LAB,GLASGOW G12 8QQ,SCOTLAND

来源：

JOURNAL OF EUKARYOTIC MICROBIOLOGY | 1994年 / 41卷 / 03期

关键词：

AMASTIGOTE; METACYCLOGENESIS; TRANSFORMATION;

D O I：

10.1111/j.1550-7408.1994.tb01497.x

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

The expression of cysteine proteinases by metacyclic promastigotes of Leishmania mexicana was investigated using gelatin polyacrylamide gel electrophoresis. Two prominent bands were detected which distinguished metacyclics from multiplicative promastigotes, lacking detectable cysteine proteinase activity, and amastigotes, with a distinct banding pattern composed of multiple enzymes. A correlation between relative activity of the metacyclic-specific bands and the prevalence of metacyclics was found both during the growth cycle in vitro as metacyclogenesis occurred, and by comparison of stationary phase populations from consecutive subpassages in vitro. Irreversible inhibition of the metacyclic activities using N-benzyloxycarbonyl-phenylalanyl-alanyl diazomethane did not inhibit metacyclic to amastigote transformation in vitro. These activities provide a useful biochemical marker for the metacyclic promastigotes of L. mexicana.

引用

页码：199 / 203

页数：5

共 12 条

[1] CHARACTERIZATION OF DEVELOPMENTALLY-REGULATED NUCLEASES IN PROMASTIGOTES AND AMASTIGOTES OF LEISHMANIA-MEXICANA [J].

BATES, PA .

FEMS MICROBIOLOGY LETTERS, 1993, 107 (01) :53-58

[2] LEISHMANIA-MEXICANA - INDUCTION OF METACYCLOGENESIS BY CULTIVATION OF PROMASTIGOTES AT ACIDIC PH [J].

BATES, PA ;

TETLEY, L .

EXPERIMENTAL PARASITOLOGY, 1993, 76 (04) :412-423

[3] AXENIC CULTIVATION AND CHARACTERIZATION OF LEISHMANIA-MEXICANA AMASTIGOTE-LIKE FORMS [J].

BATES, PA ;

ROBERTSON, CD ;

TETLEY, L ;

COOMBS, GH .

PARASITOLOGY, 1992, 105 :193-202

[4]

BATES PA, 1993, IN PRESS PARASITOLOG

[5]

Coombs G.H., 1991, P208

[6] TRANSFORMATION INVITRO OF LEISHMANIA-MEXICANA AMASTIGOTES TO PROMASTIGOTES - NUTRITIONAL-REQUIREMENTS AND THE EFFECT OF DRUGS [J].

HART, DT ;

VICKERMAN, K ;

COOMBS, GH .

PARASITOLOGY, 1981, 83 (DEC) :529-541

[7] THE LIFE-CYCLE OF LEISHMANIA IN THE SANDFLY WITH SPECIAL REFERENCE TO THE FORM INFECTIVE TO THE VERTEBRATE HOST [J].

KILLICKKENDRICK, R .

ANNALES DE PARASITOLOGIE HUMAINE ET COMPAREE, 1990, 65 :37-42

[8] ANALYSIS OF LEISHMANIA PROTEINASES REVEALS DEVELOPMENTAL-CHANGES IN SPECIES-SPECIFIC FORMS AND A COMMON 68-KDA ACTIVITY [J].

LOCKWOOD, BC ;

NORTH, MJ ;

MALLINSON, DJ ;

COOMBS, GH .

FEMS MICROBIOLOGY LETTERS, 1987, 48 (03) :345-350

[9] CHARACTERIZATION OF 3 GROUPS OF CYSTEINE PROTEINASES IN THE AMASTIGOTES OF LEISHMANIA-MEXICANA-MEXICANA [J].

ROBERTSON, CD ;

COOMBS, GH .

MOLECULAR AND BIOCHEMICAL PARASITOLOGY, 1990, 42 (02) :269-276

[10] STAGE-SPECIFIC PROTEINASES OF LEISHMANIA-MEXICANA-MEXICANA PROMASTIGOTES [J].

ROBERTSON, CD ;

COOMBS, GH .

FEMS MICROBIOLOGY LETTERS, 1992, 94 (1-2) :127-132

← 1 2 →