EFFECT OF GLUCOSE AND INSULIN IN VITRO ON UPTAKE OF TRIGLYCERIDE AND ON LIPOPROTEIN LIPASE ACTIVITY IN FAT PADS FROM NORMAL FED RATS

The effect of insulin and glucose in vitro on chylomicron triglyceride uptake and lipoprotein lipase activity was studied in epididymal fat pads of normal, fed rats. In 16 paired experiments using chylomicrons containing tritiated palmitic acid, significantly more labelled triglyceride fatty acid was found in rat fat pads when insulin and glucose were both present in the incubation mixture. Neither glucose nor insulin alone had a significant effect on triglyceride uptake. In 10 paired experiments the addition of glucose and insulin significantly raised the activity of lipoprotein lipase in fat pads. The findings support the concept that insulin enhances triglyceride removal. © 1968.