ISGF3, THE TRANSCRIPTIONAL ACTIVATOR INDUCED BY INTERFERON-ALPHA, CONSISTS OF MULTIPLE INTERACTING POLYPEPTIDE-CHAINS

被引：422

作者：

FU, XY

KESSLER, DS

VEALS, SA

LEVY, DE

DARNELL, JE

机构：

[1] ROCKEFELLER UNIV, MOLEC CELL BIOL LAB, NEW YORK, NY 10021 USA

[2] NYU MED CTR, DEPT PATHOL, NEW YORK, NY 10016 USA

[3] NYU MED CTR, KAPLAN CANC CTR, NEW YORK, NY 10016 USA

来源：

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA | 1990年 / 87卷 / 21期

关键词：

factor purification; interferon-dependent transcription factor; interferon-stimulated response element;

D O I：

10.1073/pnas.87.21.8555

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Interferon-stimulated gene factor 3 (ISGF3) is the ligand-dependent transcriptional activator that, in response to interferon treatment, is assembled in the cell cytoplasm, is translocated to the nucleus, and binds the consensus DNA site, the interferon-stimulated response element. We have purified ISGF3 and identified its constituent proteins: a DNA-binding protein of 48 kDa and three larger polypeptides (84, 91, and 113 kDa), which themselves do not have DNA-binding activity. The multisubunit structure of ISGF3 most likely reflects its participation in receiving a ligand-dependent signal, translocating to the nucleus, and binding to DNA to activate transcription.

引用

页码：8555 / 8559

页数：5

共 28 条

[1] ACTIVATION OF DNA-BINDING ACTIVITY IN AN APPARENTLY CYTOPLASMIC PRECURSOR OF THE NF-KAPPA-B TRANSCRIPTION FACTOR [J].