THE ADE6-GENE OF THE FISSION YEAST SCHIZOSACCHAROMYCES-POMBE HAS THE SAME CHROMATIN STRUCTURE IN THE CHROMOSOME AND IN PLASMIDS

被引:30
作者
BERNARDI, F [1 ]
KOLLER, T [1 ]
THOMA, F [1 ]
机构
[1] SWISS FED INST TECHNOL, INST ZELLBIOL, CH-8093 ZURICH, SWITZERLAND
关键词
CHROMATIN; YEAST; SCHIZOSACCHAROMYCES-POMBE; ADE6-GENE;
D O I
10.1002/yea.320070603
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
We have analysed the chromatin structure of the ade6 gene of Schizosaccharomyces pombe and its flanking regions both in the chromosome and in plasmids. The chromatin structure is independent of the chromosomal or extra-chromosomal location. The ade6 gene contains eight precisely positioned nucleosomes on the 5' half, 'not positioned' nucleosomes around the 3' end and a nuclease-sensitive promoter region. Precisely positioned nucleosomes, but no nuclease-sensitive region were also detected on the ura4 gene in the chromosome and on a plasmid. The results show that S. pombe chromosomal and extrachromosomal genes have chromatin structures similar to those of S. cerevisiae and higher eukaryotes.
引用
收藏
页码:547 / 558
页数:12
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