BIOLOGICAL CLONING OF FUNCTIONALLY DIVERSE QUASI-SPECIES OF HIV-1

被引：24

作者：

CONNOR, RI ^{[1
]}

NOTERMANS, DW ^{[1
]}

MOHRI, H ^{[1
]}

CAO, YZ ^{[1
]}

HO, DD ^{[1
]}

机构：

[1] NYU,SCH MED,AARON DIAMOND AIDS RES CTR,455 1ST AVE,NEW YORK,NY 10016

来源：

AIDS RESEARCH AND HUMAN RETROVIRUSES | 1993年 / 9卷 / 06期

关键词：

D O I：

10.1089/aid.1993.9.541

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

Twenty putative clones of HIV-1, derived by biological cloning from two different individuals, were characterized in vitro for functional activity and genotypic diversity. Many of the biological clones isolated by this method were genetically distinct, and could be further distinguished by differences in replication kinetics, host cell range, and/or cytopathicity. Our findings indicate that biological cloning by limiting dilution cultures is an effective method for isolating replication-competent, functionally diverse quasispecies of HIV-1.

引用

页码：541 / 546

页数：6

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