CHARACTERIZATION OF THE DEFECT IN THE ESCHERICHIA-COLI MUTT1 MUTATOR GENE

被引：10

作者：

BHATNAGAR, SK

BULLIONS, LC

LEW, G

BESSMAN, MJ

机构：

[1] JOHNS HOPKINS UNIV,MCCOLLUM PRATT INST,BALTIMORE,MD 21218

[2] JOHNS HOPKINS UNIV,DEPT BIOL,BALTIMORE,MD 21218

来源：

JOURNAL OF BACTERIOLOGY | 1990年 / 172卷 / 05期

关键词：

D O I：

10.1128/jb.172.5.2802-2803.1990

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

With a probe constructed from the wild-type gene, a DNA fragment containing the entire mutT1 mutator gene was isolated and cloned into pUC18. Nucleotide sequence analysis revealed that the mutator defect was most likely due to an IS1 insertion into the wild-type gene.

引用

页码：2802 / 2803

页数：2

共 14 条

[1] MOLECULAR-CLONING AND NUCLEOTIDE-SEQUENCE OF THE MUTT-MUTATOR OF ESCHERICHIA-COLI THAT CAUSES A-T TO C-G TRANSVERSION [J].

AKIYAMA, M ;

HORIUCHI, T ;

SEKIGUCHI, M .

MOLECULAR & GENERAL GENETICS, 1987, 206 (01) :9-16

[2] A SPECIFIC ROLE OF MUTT PROTEIN - TO PREVENT DG.DA MISPAIRING IN DNA-REPLICATION [J].

AKIYAMA, M ;

MAKI, H ;

SEKIGUCHI, M ;

HORIUCHI, T .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1989, 86 (11) :3949-3952

[3] SCREENING GAMMAGT RECOMBINANT CLONES BY HYBRIDIZATION TO SINGLE PLAQUES INSITU [J].

BENTON, WD ;

DAVIS, RW .

SCIENCE, 1977, 196 (4286) :180-182

[4]

BHATNAGAR SK, 1988, J BIOL CHEM, V263, P8953

[5] IS1 INSERTION GENERATES DUPLICATION OF A 9 BASE PAIR SEQUENCE AT ITS TARGET SITE [J].

GRINDLEY, NDF .

CELL, 1978, 13 (03) :419-426

[6] Variant insertion element IS1 generates 8-base pair duplications of the target sequence [J].

Iida, Shigeru ;

Marcoli, Roberto ;

Bickle, Thomas A. .

NATURE, 1981, 294 (5839) :374-376

[7]

Maniatis T., 1982, MOL CLONING

[8] NUCLEOTIDE-SEQUENCE OF AN INSERTION ELEMENT, IS1 [J].

OHTSUBO, H ;

OHTSUBO, E .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1978, 75 (02) :615-619

[9] STUDY OF A SUPPRESSOR OF MUTT IN ESCHERICHIA-COLI [J].

RAY, U .

MOLECULAR & GENERAL GENETICS, 1979, 171 (02) :215-218

[10] MODEL FOR THE PARTICIPATION OF QUASI-PALINDROMIC DNA-SEQUENCES IN FRAMESHIFT MUTATION [J].

RIPLEY, LS .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES, 1982, 79 (13) :4128-4132

← 1 2 →