CLONING OF THE YEAST SFL2 GENE - ITS DISRUPTION RESULTS IN PLEIOTROPIC PHENOTYPES CHARACTERISTIC FOR TUP1 MUTANTS

被引：41

作者：

FUJITA, A

MATSUMOTO, S

KUHARA, S

MISUMI, Y

KOBAYASHI, H

机构：

[1] FUKUOKA UNIV,SCH MED,DEPT BIOCHEM,MINAMI KU,FUKUOKA 813,JAPAN

[2] GODO SHUSEI CORP,SHIZUOKA 424,JAPAN

[3] KYUSHU UNIV,FAC AGR,DEPT AGR CHEM,HIGASHI KU,FUKUOKA 812,JAPAN

来源：

GENE | 1990年 / 89卷 / 01期

关键词：

flocculation; nucleotide sequence; Recombinant DNA; Saccharomyces cerevisiae;

D O I：

10.1016/0378-1119(90)90210-I

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

We have identified a yeast gene, SFL2 (suppressor gene for flocculation) which complemented a newly isolated sfl2 mutant. This mutation causes asexual cell aggregation. The strain bearing the SFL2 gene disruption exhibited pleiotropic phenotypes characteristics for tup1 mutants. Physical mapping and complementation analysis suggested that the cloned SFL2 gene is identical to the TUP1 gene. The SFL2 gene encodes a 669-amino acid protein which has domains rich in glutamine, as does the SSN6 protein. © 1990.

引用

页码：93 / 99

页数：7

共 34 条

[1] STERILE HOST YEASTS (SHY) - EUKARYOTIC SYSTEM OF BIOLOGICAL CONTAINMENT FOR RECOMBINANT DNA EXPERIMENTS [J].