PURIFICATION OF THE CYTOCHALASIN-B BINDING-COMPONENT OF THE HUMAN-ERYTHROCYTE MONOSACCHARIDE TRANSPORT-SYSTEM

被引:108
作者
BALDWIN, SA
BALDWIN, JM
GORGA, FR
LIENHARD, GE
机构
[1] Department of Biochemistry, Dartmouth Medical School, Hanover
关键词
(Human erythrocyte); Cytochalasin B binding component; Monosaccharide transport;
D O I
10.1016/0005-2736(79)90257-8
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The cytochalasin B binding component of the human erythrocyte monosaccharide transport system has been purified. The preparation appears to contain one major protein with an apparent polypeptide chain molecular weight of 55 000 and about 0.4 binding sites per chain. Cytochalasin B binds to the reconstituted preparation with a dissociation constant of 1.3·10-7 M, a value which is similar to that reported for the transport system in the intact erythrocyte. © 1979.
引用
收藏
页码:183 / 188
页数:6
相关论文
共 18 条
[1]  
BATT ER, 1976, J BIOL CHEM, V251, P7184
[2]  
DRICKAMER LK, 1978, FED PROC, V37, P1508
[3]   SIMPLE PROCEDURE FOR REMOVAL OF TRITON X-100 FROM PROTEIN SAMPLES [J].
HOLLOWAY, PW .
ANALYTICAL BIOCHEMISTRY, 1973, 53 (01) :304-308
[4]  
JUNG CY, 1977, J BIOL CHEM, V252, P5456
[5]  
KAGAWA Y, 1971, J BIOL CHEM, V246, P5477
[6]   RECONSTITUTION OF D-GLUCOSE TRANSPORT IN VESICLES COMPOSED OF LIPIDS AND INTRINSIC PROTEIN (ZONE 4 5) OF HUMAN ERYTHROCYTE-MEMBRANE [J].
KAHLENBERG, A ;
ZALA, CA .
JOURNAL OF SUPRAMOLECULAR STRUCTURE, 1977, 7 (3-4) :287-300
[7]  
KASAHARA M, 1977, J BIOL CHEM, V252, P7384
[8]   MONOSACCHARIDE TRANSPORT-SYSTEM OF HUMAN ERYTHROCYTE - IDENTIFICATION OF CYTOCHALASIN-B BINDING COMPONENT [J].
LIENHARD, GE ;
GORGA, FR ;
ORASKY, JE ;
ZOCCOLI, MA .
BIOCHEMISTRY, 1977, 16 (22) :4921-4926
[9]  
LIN S, 1977, J BIOL CHEM, V252, P5464
[10]  
LIN S, 1974, J BIOL CHEM, V249, P5778