A HIGHLY CONSERVED TYROSINE RESIDUE AT CODON 845 WITHIN THE KINASE DOMAIN IS NOT REQUIRED FOR THE TRANSFORMING ACTIVITY OF HUMAN EPIDERMAL GROWTH-FACTOR RECEPTOR

被引：112

作者：

GOTOH, N

TOJO, A

HINO, M

YAZAKI, Y

SHIBUYA, M

机构：

[1] UNIV TOKYO,INST MED SCI,DEPT GENET,4-6-1 SHIROKANEDAI MINATO KU,TOKYO 108,JAPAN

[2] UNIV TOKYO,INST MED SCI,DEPT HEMATOL ONCOL,MINATO KU,TOKYO 108,JAPAN

[3] OSAKA CITY UNIV,SCH MED,DEPT INTERNAL MED 2,ABENO KU,OSAKA 545,JAPAN

[4] UNIV TOKYO,FAC MED,DEPT INTERNAL MED 3,BUNKYO KU,TOKYO 103,JAPAN

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1992年 / 186卷 / 02期

关键词：

D O I：

10.1016/0006-291X(92)90812-Y

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Epidermal growth factor receptor (EGF-R) is a widely expressed ligand-dependent tyrosine kinase. The tyrosine residue at 845 in EGF-R corresponds to Y416 of v c-src kinase, which is highly conserved and functionally important in many tyrosine kinases. To clarify the functional role of Y845, we constructed a mutant human EGF-R in which this tyrosine was replaced with phenylalanine and transfected it to NIH3T3 cells. EGF-R F845 induced EGF-dependent cellular transformation and revealed tyrosine-autophosphorylation of a 170kDa protein, and initiated DNA synthesis similar to the wild-type EGF-R. We conclude here that Y845 is dispensable in the above mentioned functions of EGF-R tyrosine kinase. © 1992.

引用

页码：768 / 774

页数：7

共 28 条

[1]

BERTICS PJ, 1985, J BIOL CHEM, V260, P4642

[2]

BERTICS PJ, 1988, J BIOL CHEM, V263, P3610

[3] FUNCTIONAL INDEPENDENCE OF THE EPIDERMAL GROWTH-FACTOR RECEPTOR FROM A DOMAIN REQUIRED FOR LIGAND-INDUCED INTERNALIZATION AND CALCIUM REGULATION [J].