SPECIFIC BINDING OF INFLUENZA-A VIRUS NS1 PROTEIN TO THE VIRUS MINUS-SENSE RNA INVITRO

被引:57
作者
HATADA, E [1 ]
TAKIZAWA, T [1 ]
FUKUDA, R [1 ]
机构
[1] KANAZAWA UNIV,SCH MED,DEPT BIOCHEM,KANAZAWA,ISHIKAWA 920,JAPAN
关键词
D O I
10.1099/0022-1317-73-1-17
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
The non-structural protein NS1, encoded by genome segment 8 of influenza A virus, was expressed in Escherichia coli from cloned cDNA and purified. The NS1 protein had a specific RNA-binding activity, binding to influenza A virus minus-sense but not plus-sense RNA synthesized in vitro from cloned DNA using phage RNA polymerase. NS1 bound preferentially to the regions of RNA containing either 5'- or 13'-terminal common sequences of the genomic RNA. Binding was inhibited by virion RNA, but not by, single-stranded minus-sense cDNA and oligo DNAs having the common sequences. In addition, binding was also inhibited by 28S rRNA but not 18S rRNA prepared from MDCK cells.
引用
收藏
页码:17 / 25
页数:9
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