STEPWISE ACCELERATION OF PHOSPHATIDYL CHOLINE SYNTHESIS IN PHYTOHEMAGGLUTININ-TREATED LYMPHOCYTES

被引：42

作者：

FISHER, DB

MUELLER, GC

机构：

[1] McArdle Laboratory, University of Wisconsin, Madison

来源：

BIOCHIMICA ET BIOPHYSICA ACTA | 1969年 / 176卷 / 02期

关键词：

D O I：

10.1016/0005-2760(69)90189-1

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Phytohemagglutinin accelerates the incorporation of labeled choline into total lymphocyte lipid(> 95% in phosphatidyl choline) in a stepwise manner. The early acceleration of choline incorporation (0-2 h) is independent of protein synthesis while the late acceleration (24-48 h) is dependent upon protein synthesis. The labeling of phosphatidyl choline by [Me-14C]methionine is also accelerated by phytohemagglutinin. Phytohemagglutinin stimulates the accumulation first of phosphatidyl ethanolamine and phosphatidyl serine (0-24 h), then of phosphatidyl choline and phosphatidyl inositol (24-48 h). A model is presented for the roles that these stepwise alterations in phospholipid metabolism play in growth induction by phytohemagglutinin. © 1969.

引用

页码：316 / &

共 18 条

[1] ISOLATION AND CHARACTERIZATION OF A NOVEL MICROSOME FRACTION FROM WASHED AND DETERGENT-TREATED NUCLEI OF HELA CELLS BY USE OF SOLUTIONS CONTAINING DEOXYRIBONUCLEIC ACID [J].