MICROHETEROGENEITY OF MAMMALIAN HAPTOGLOBINS IN ISOELECTRIC-FOCUSING

被引：9

作者：

DOBRYSZYCKA, W

KRAWCZYK, E

机构：

[1] Department of Clinical Investigation, Bio-Pharmaceutical Institute, Medical Academy of Wrocław

来源：

COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY | 1979年 / 62卷 / 01期

关键词：

D O I：

10.1016/0305-0491(79)90022-1

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

1. 1. Human haptoglobin type 1-1, porcine haptoglobin, and equine haptoglobin were isolated and purified. 2. 2. These haptoglobins were similar in polyacrylamide gel electrophoresis and in subunit structure but showed microheterogeneity in isoelectric focusing. 3. 3. Isoelectric points of human haptoglobin as determined with photopolymerized gels were found to be 4.03-4.24, of porcine haptoglobin 4.0-4.30, and of horse haptoglobin 3.80-4.15, respectively. 4. 4. Results obtained with chemically polymerized gels were 0.08-0.3 pH units higher. 5. 5. Examined haptoglobins differed also in the ability of complex formation with hemoglobin, in sialic acid content and in antigenic specificity. © 1979.

引用

页码：111 / 113

页数：3

共 18 条

[1]

BELOSTOTZKI WM, 1973, BIOKHIMIYA, V38, P733

[2] PURIFICATION OF HAPTOGLOBIN [J].

CONNELL, GE ;

SHAW, RW .

CANADIAN JOURNAL OF BIOCHEMISTRY AND PHYSIOLOGY, 1961, 39 (06) :1013-&

[3]

DOBRYSZYCKA W, 1977, INT J BIOCHEM, V8, P511, DOI 10.1016/0020-711X(77)90113-6

[4] STUDIES ON BINDING OF HEMOGLOBIN BY HAPTOGLOBIN USING ELECTROFOCUSING AND GRADIENT ELECTROPHORESIS [J].

EMES, AV ;

LATNER, AL ;

MARTIN, JA ;

MULLIGAN, FA .

BIOCHIMICA ET BIOPHYSICA ACTA, 1976, 420 (01) :57-68

[5] STUDIES ON PORCINE HAPTOGLOBIN AND ITS COMPLEX WITH HUMAN HEMOGLOBIN [J].

FRASER, IH ;

SMITH, DB .

CANADIAN JOURNAL OF BIOCHEMISTRY, 1971, 49 (02) :141-&

[6]

HAGLUND H, 1971, METHOD BIOCHEM ANAL, V19, P82

[7]

JAVID J, 1971, AM J HUM GENET, V23, P496

[8]

JAYLE MF, 1951, B SOC CHIM BIOL, V33, P876

[9] STUDIES ON DISSOCIATION OF PORCINE HAPTOGLOBIN [J].

LOCKHART, WL ;

SMITH, DB ;

CHUNG, WP .

CANADIAN JOURNAL OF BIOCHEMISTRY, 1972, 50 (07) :775-+

[10] HALF-MOLECULE OF HAPTOGLOBIN - STUDIES ON PRODUCT OBTAINED BY SELECTIVE CLEAVAGE OF A HAPTOGLOBIN DISULFIDE [J].

MALCHY, B ;

RORSTAD, O ;

DIXON, GH .

CANADIAN JOURNAL OF BIOCHEMISTRY, 1973, 51 (03) :265-273

← 1 2 →