BIOCHEMICAL-PROPERTIES OF HISTONE-LIKE PROTEINS OF PROCYCLIC TRYPANOSOMA-BRUCEI-BRUCEI

被引：8

作者：

BENDER, K

BETSCHART, B

SCHALLER, J

KAMPFER, U

HECKER, H ^{[1
]}

机构：

[1] SWISS TROP INST, CH-4002 BASEL, SWITZERLAND

[2] INST BIOCHEM, BERN, SWITZERLAND

来源：

ACTA TROPICA | 1991年 / 50卷 / 02期

关键词：

TRYPANOSOME; TRYPANOSOMA-BRUCEI BRUCEI; CHROMATIN; HISTONE; PROTEIN-ELECTROPHORESIS; REVERSED PHASE CHROMATOGRAPHY; AMINO ACID ANALYSIS;

D O I：

10.1016/0001-706X(91)90010-H

中图分类号：

R38 [医学寄生虫学]; Q [生物科学];

学科分类号：

07 ; 0710 ; 09 ; 100103 ;

摘要：

Four histone-like proteins a, b, c, d were extracted with 0.2 M H2SO4 from soluble nuclear chromatin of Trypanosoma brucei brucei procyclic culture forms and purified by FPLC reversed phase chromatography. The amino acid composition of these proteins and their electrophoretic mobilities in three different gel systems strongly indicated their core histone nature. Similarities were found between a, b, c and d with the core histones H3, H2A, H2B and H4 of higher eukaryotes, respectively. On the other hand, these proteins also showed differences as compared to higher eukaryotes; proteins a and d clearly differed from their counterparts H3 and H4 on the basis of their hydrophobic properties. The results indicate the occurrence of core histone variants in T.b. brucei which may influence DNA-histone and histone-histone interactions as well as the chromatin compaction in the nucleus of this protozoan parasite.

引用

页码：169 / 184

页数：16

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