MOLECULAR-CLONING OF 2 GLUTAMATE TRANSPORTER SUBTYPES FROM MOUSE-BRAIN

被引：40

作者：

MUKAINAKA, Y ^{[1
]}

TANAKA, K ^{[1
]}

HAGIWARA, T ^{[1
]}

WADA, K ^{[1
]}

机构：

[1] NATL CTR NEUROL & PSYCHIAT,NATL INST NEUROSCI,DEPT DEGENERAT NEUROL DIS,KODAIRA,TOKYO 187,JAPAN

来源：

BIOCHIMICA ET BIOPHYSICA ACTA-GENERAL SUBJECTS | 1995年 / 1244卷 / 01期

关键词：

MOLECULAR CLONING; GLUTAMATE TRANSPORTER; MOUSE BRAIN; CDNA SEQUENCE;

D O I：

10.1016/0304-4165(95)00062-G

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The physiological action of glutamate is terminated by diffusional processes with its subsequent removal by high-affinity transport systems localized to glial and/or neuronal elements. Several cDNAs encoding glutamate transporters have been isolated from mammalian tissues. Here, we screened a cDNA library derived from mouse cerebellum and isolated the two glutamate transporters, termed MGLT1 and MEAAC1. The MGLT1 and MEAAC1 cDNAs encode proteins of 572 and 523 amino acids, respectively. MGLT1 has 93.9% amino acid sequence identity with the rat GLT1 and MEAAC1 has 89.3% identity with the rabbit EAAC1. MEAAC1 mRNA was expressed in brain, lung, kidney and skeletal muscle, whereas expression of MGLT1 was restricted to brain.

引用

页码：233 / 237

页数：5

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