APOPTOSIS DURING RETINOIC ACID-INDUCED DIFFERENTIATION OF F9 EMBRYONAL CARCINOMA-CELLS

被引：57

作者：

ATENCIA, R ^{[1
]}

GARCIASANZ, M ^{[1
]}

UNDA, F ^{[1
]}

ARECHAGA, J ^{[1
]}

机构：

[1] UNIV BASQUE COUNTRY, FAC MED, DEPT BIOL CELULAR, E-48940 VIZCAYA, SPAIN

来源：

EXPERIMENTAL CELL RESEARCH | 1994年 / 214卷 / 02期

关键词：

D O I：

10.1006/excr.1994.1304

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

In this work we have characterized a process of cell death by apoptosis occurring in retinoic acid-induced differentiation of F9 embryonal carcinoma cells. Morphological changes corresponding to apoptosis were observed concomitantly with those of differentiated phenotype, in cell cultures treated for 48 h with physiological concentrations of all-trans-retinoic acid. Internucleosomal DNA fragmentation was also detected using agarose gels. These observations show that retinoic acid can induce both differentiation and apoptosis in an experimental system-the teratocarcinoma stem cell lines widely used so far in the study of differentiation. We present this system as a tool for a better understanding of the role of retinoic acid in the regulation of cell differentiation and programmed cell death. (C) 1994 Academic Press, Inc.

引用

页码：663 / 667

页数：5

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