MOLECULAR-CLONING AND DEVELOPMENTAL EXPRESSION OF THE RAT CARDIAC-SPECIFIC ISOFORM OF TROPONIN-I

被引：33

作者：

MARTIN, AF ^{[1
]}

ORLOWSKI, J ^{[1
]}

机构：

[1] UNIV CINCINNATI, COLL MED, DEPT MOLEC GENET BIOCHEM & MICROBIOL, CINCINNATI, OH 45267 USA

来源：

JOURNAL OF MOLECULAR AND CELLULAR CARDIOLOGY | 1991年 / 23卷 / 05期

关键词：

TROPONIN-I; CARDIAC; CDNA CLONE; MESSENGER RNA; DEVELOPMENTAL EXPRESSION;

D O I：

10.1016/0022-2828(91)90050-V

中图分类号：

R5 [内科学];

学科分类号：

1002 ; 100201 ;

摘要：

Troponin I is the subunit of the troponin complex in striated muscle which inhibits actomyosin ATPase activity. We have isolated a full-length cDNA clone for rat cardiac troponin I and determined its nucleic acid sequence. The amino acid sequence deduced from this clone shows 88%-92% similarity with previously reported amino acid sequences for rabbit (Wilkinson and Grand, 1978) and bovine (Leszyk et al.) cardiac troponin I. Examination of cardiac troponin I mRNA abundance during development revealed a 15-fold induction in its expression in the adult heart compared to that in embryonic (14 day) heart muscle. Furthermore, expression of cardiac troponin I mRNA was restricted to heart muscle and was not detected in skeletal muscle at any developmental stage. © 1991.

引用

页码：583 / 588

页数：6

共 29 条

[1] STRUCTURE, EVOLUTION, AND REGULATION OF A FAST SKELETAL-MUSCLE TROPONIN-I GENE [J].

BALDWIN, AS ;

KITTLER, ELW ;

EMERSON, CP .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1985, 82 (23) :8080-8084

[2] ISOLATION AND CHARACTERIZATION OF A CDNA CLONE FOR HUMAN FERRITIN HEAVY-CHAIN [J].

BOYD, D ;

JAIN, SK ;

CRAMPTON, J ;

BARRETT, KJ ;

DRYSDALE, J .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES, 1984, 81 (15) :4751-4755

[3]

CHIZZONITE RA, 1984, J BIOL CHEM, V259, P2628

[4]

CHOMCZYNSKI P, 1987, ANAL BIOCHEM, V162, P156, DOI 10.1016/0003-2697(87)90021-2

[5] STUDIES ON PHOSPHORYLATION OF INHIBITORY SUBUNIT OF TROPONIN DURING MODIFICATION OF CONTRACTION IN PERFUSED RAT-HEART [J].

ENGLAND, PJ .

BIOCHEMICAL JOURNAL, 1976, 160 (02) :295-304

[6] A TECHNIQUE FOR RADIOLABELING DNA RESTRICTION ENDONUCLEASE FRAGMENTS TO HIGH SPECIFIC ACTIVITY [J].

FEINBERG, AP ;

VOGELSTEIN, B .

ANALYTICAL BIOCHEMISTRY, 1983, 132 (01) :6-13

[7]

GUSTAFSON TA, 1987, J BIOL CHEM, V262, P13316

[8] ELECTROPHORETIC ANALYSIS OF MULTIPLE FORMS OF RAT CARDIAC MYOSIN - EFFECTS OF HYPOPHYSECTOMY AND THYROXINE REPLACEMENT [J].

HOH, JFY ;

MCGRATH, PA ;

HALE, PT .

JOURNAL OF MOLECULAR AND CELLULAR CARDIOLOGY, 1978, 10 (11) :1053-+

[9] ALL MEMBERS OF THE MHC MULTIGENE FAMILY RESPOND TO THYROID-HORMONE IN A HIGHLY TISSUE-SPECIFIC MANNER [J].

IZUMO, S ;

NADALGINARD, B ;

MAHDAVI, V .

SCIENCE, 1986, 231 (4738) :597-600

[10]

KOPPE RI, 1989, J BIOL CHEM, V264, P14327

← 1 2 3 →