SOLUBLE CD40 LIGAND CAN REPLACE THE NORMAL T-CELL-DERIVED CD40 LIGAND SIGNAL TO B-CELLS IN T-CELL-DEPENDENT ACTIVATION

被引：283

作者：

LANE, P

BROCKER, T

HUBELE, S

PADOVAN, E

LANZAVECCHIA, A

MCCONNELL, F

机构：

[1] Basel Institute for Immunology, Basel

来源：

JOURNAL OF EXPERIMENTAL MEDICINE | 1993年 / 177卷 / 04期

关键词：

D O I：

10.1084/jem.177.4.1209

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

We have constructed a soluble chimeric fusion protein between the mouse CD8alpha chain and the mouse CD40 T cell ligand. This protein binds to both human and mouse B cells. By itself it induced a modest degree of B cell proliferation, but together with antiimmunoglobulin (anti-Ig) antibody it greatly stimulated B cell proliferation, as determined by both [H-3]thymidine uptake and increase in cell numbers. These data are evidence that the CD40 ligand on T cells provides a signal that drives B cell proliferation. This signal is synergistic with that delivered by anti-Ig antibody.

引用

收藏

页码：1209 / 1213

页数：5

相关论文

共 16 条

[1] MOLECULAR AND BIOLOGICAL CHARACTERIZATION OF A MURINE LIGAND FOR CD40 [J].

ARMITAGE, RJ ;

FANSLOW, WC ;

STROCKBINE, L ;

SATO, TA ;

CLIFFORD, KN ;

MACDUFF, BM ;

ANDERSON, DM ;

GIMPEL, SD ;

DAVISSMITH, T ;

MALISZEWSKI, CR ;

CLARK, EA ;

SMITH, CA ;

GRABSTEIN, KH ;

COSMAN, D ;

SPRIGGS, MK .

NATURE, 1992, 357 (6373) :80-82

[2] STIMULATION OF B-CELL PROLIFERATION BY MEMBRANE-ASSOCIATED MOLECULES FROM ACTIVATED T-CELLS [J].

BRIAN, AA .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1988, 85 (02) :564-568

[3] A NEW MEMBER OF THE IMMUNOGLOBULIN SUPERFAMILY - CTLA-4 [J].

BRUNET, JF ;

DENIZOT, F ;

LUCIANI, MF ;

ROUXDOSSETO, M ;

SUZAN, M ;

MATTEI, MG ;

GOLSTEIN, P .

NATURE, 1987, 328 (6127) :267-270

[4] ACTIVATION OF HUMAN B-CELLS MEDIATED THROUGH 2 DISTINCT CELL-SURFACE DIFFERENTIATION ANTIGENS, BP35 AND BP50 [J].

CLARK, EA ;

LEDBETTER, JA .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1986, 83 (12) :4494-4498

[5] THE HINGE REGION OF THE CD8-ALPHA CHAIN - STRUCTURE, ANTIGENICITY, AND UTILITY IN EXPRESSION OF IMMUNOGLOBULIN SUPERFAMILY DOMAINS [J].

CLASSON, BJ ;

BROWN, MH ;

GARNETT, D ;

SOMOZA, C ;

BARCLAY, AN ;

WILLIS, AC ;

WILLIAMS, AF .

INTERNATIONAL IMMUNOLOGY, 1992, 4 (02) :215-225

[6]

GORDON J, 1988, J IMMUNOL, V140, P1425

[7] A HUMAN BETA-ACTIN EXPRESSION VECTOR SYSTEM DIRECTS HIGH-LEVEL ACCUMULATION OF ANTISENSE TRANSCRIPTS [J].

GUNNING, P ;

LEAVITT, J ;

MUSCAT, G ;

NG, SY ;

KEDES, L .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1987, 84 (14) :4831-4835

[8]

HIROHATA S, 1988, J IMMUNOL, V140, P3726

[9] ACTIVATED HUMAN T-CELLS EXPRESS A LIGAND FOR THE HUMAN-B CELL-ASSOCIATED ANTIGEN CD40 WHICH PARTICIPATES IN T-CELL-DEPENDENT ACTIVATION OF LYMPHOCYTES-B [J].

LANE, P ;

TRAUNECKER, A ;

HUBELE, S ;

INUI, S ;

LANZAVECCHIA, A ;

GRAY, D .

EUROPEAN JOURNAL OF IMMUNOLOGY, 1992, 22 (10) :2573-2578

[10] ACTIVATION OF HUMAN LYMPHOCYTES-B - FREQUENCY OF ANTIGEN-SPECIFIC B-CELLS TRIGGERED BY ALLOREACTIVE OR BY ANTIGEN-SPECIFIC T-CELL CLONES [J].

LANZAVECCHIA, A ;

PARODI, B ;

CELADA, F .

EUROPEAN JOURNAL OF IMMUNOLOGY, 1983, 13 (09) :733-738