CREATION OF MYELOMENINGOCELE IN-UTERO - A MODEL OF FUNCTIONAL DAMAGE FROM SPINAL-CORD EXPOSURE IN FETAL SHEEP

被引:190
作者
MEULI, M
MEULISIMMEN, C
YINGLING, CD
HUTCHINS, GM
HOFFMAN, KM
HARRISON, MR
ADZICK, NS
机构
[1] UNIV CALIF SAN FRANCISCO, CTR FETAL TREATMENT, DEPT ANESTHESIA, SAN FRANCISCO, CA 94143 USA
[2] UNIV CALIF SAN FRANCISCO, ANIM CARE FACIL, SAN FRANCISCO, CA 94143 USA
[3] DAVIES MED CTR, MICROSURG LAB, SAN FRANCISCO, CA USA
[4] JOHNS HOPKINS MED INST, DEPT PATHOL, BALTIMORE, MD 21205 USA
关键词
MYELOMENINGOCELE; NEURAL TUBE DEFECT; SPINA BIFIDA; SPINAL DYSRAPHIA; FETAL SURGERY;
D O I
10.1016/0022-3468(95)90335-6
中图分类号
R72 [儿科学];
学科分类号
100202 ;
摘要
A recent study in human fetuses with myelomeningocele (MMC) suggested that the primary malformation is not neural but a failed closure of the posterior vertebral column and paraspinal soft tissue, which reads to exposure and secondary destruction of the spinal cord. The goal of this study was to test whether chronic exposure of the normal spinal cord to the amniotic space produces a lesion similar to human MMC. In fetal sheep at 75 days' gestation (group A) and 60 days' gestation (group B) (term = 150 days), the lumbar spinal cord was exposed to the amniotic cavity by excising skin and paraspinal soft tissues, and by performing a laminectomy. Some animals from both groups were fetectomized and assessed morphologically at 100 days' gestation. The remainder were delivered near term and assessed clinically, electrophysiologically, and morphologically. In group A, all animals showed MMC type pathology. The exposed spinal cord was herniated out of the spinal canal and rested on the dorsal membranes of a cystic sac. The neural tissue was stretched and flattened out. Histologically, the hallmarks of the spinal cord were not discernable and the cytoarchitecture was lost. These changes were less severe at 100 days than at term. The three survivors in group A were paraplegic. In group a, the two survivors and two fetuses harvested at 100 days had healed skin wounds and near normal spinal cord histology. The other animal harvested at 100 days had a MMC-type lesion with less severe histological changes. The two survivors had a mild paraparesis. In conclusion, surgical exposure of the normal spinal cord to the amniotic space in a 75 day sheep fetus results in a MMC-type pathology at birth, which clinically and morphologically resembles human MMC. The creation of a spine bifida-type lesion in a 60-day-old fetus may result in spontaneous healing and minimal neurological deficit at birth. This ''healing experiment of nature'' suggests that in utero repair of MMC might prevent spinal cord damage and spare neurological function. Copyright (C) 1995 by W.B. Saunders Company
引用
收藏
页码:1028 / 1033
页数:6
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