OPTICAL ANALYSIS OF SYNAPTIC VESICLE RECYCLING AT THE FROG NEUROMUSCULAR-JUNCTION

被引：646

作者：

BETZ, WJ

BEWICK, GS

机构：

[1] Department of Physiology, University of Colorado School of Medicine, Denver

[2] Muscular Dystrophy Research Laboratories, Newcastle General Hospital, Newcastle-Upon-Tyne

来源：

SCIENCE | 1992年 / 255卷 / 5041期

关键词：

D O I：

10.1126/science.1553547

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

The fluorescent dyes FM1-43 and RH414 label motor nerve terminals in an activity-dependent fashion that involves dye uptake by synaptic vesicles that are recycling. This allows optical monitoring of vesicle recycling in living nerve terminals to determine how recycled vesicles reenter the vesicle pool. The results suggest that recycled vesicles mix with the pool morphologically and functionally. One complete cycle of release of transmitter, recycling of a vesicle, and rerelease of transmitter appears to take about 1 minute.

引用

页码：200 / 203

页数：4

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