PROTEIN BLOTTING FOLLOWED BY MICROSEQUENCING

被引：22

作者：

CHOLI, T

WITTMANNLIEBOLD, B

机构：

[1] Max-Planck Institut Für Molekulare Genetik, Berlin, D-1000 33, Abteilung Wittmann, Dahlem

来源：

ELECTROPHORESIS | 1990年 / 11卷 / 07期

关键词：

D O I：

10.1002/elps.1150110706

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

The use of new membranes such as activated or derivatized glass fibers as well as synthetic membranes, which are compatible with the hazardous sequencing reagents, are described. Precautions to be taken in order to prevent N‐terminal block age of the proteins during electrophoresis and blotting are described, as well as the conditions for protein detection after blotting and protein treatment for in situ amino acid analysis, fragmentation and microsequencing. For a number of standard proteins and bacterial ribosomal proteins microsequence analysis is reported for two commercially available sequencers (Applied Biosystems and Knauer). Copyright © 1990 VCH Verlagsgesellschaft mbH

引用

页码：562 / 568

页数：7

共 81 条

[1] INTERNAL AMINO-ACID SEQUENCE-ANALYSIS OF PROTEINS SEPARATED BY ONE-DIMENSIONAL OR TWO-DIMENSIONAL GEL-ELECTROPHORESIS AFTER INSITU PROTEASE DIGESTION ON NITROCELLULOSE [J].