INHIBITION OF ACTIVITY OF ENCEPHALOMYOCARDITIS VIRUS-INDUCED RNA-POLYMERASE BY ANTIBODIES AGAINST CELLULAR-COMPONENTS

Antibodies raised against a fraction of homogenate of uninfected Krebs II cells are able to inhibit the RNA polymerase activity of replication complexes induced upon infection of these cells with encephalomyocarditis virus. The inhibitory activity of these antibodies can be absorbed by the cellular fraction used for immunization. When tested with separated replication complexes synthesizing preferentially either single- or double-stranded virus-specific RNA, the anti-host antibodies inhibit the formation of single-stranded RNA totally and the synthesis of double-stranded RNA partially. The results are consistent with a notion that a host cell component(s) is a functionally important part of viral replication complexes. © 1979.