STRUCTURE-INDEPENDENT NUCLEOTIDE-SEQUENCE ANALYSIS

被引：204

作者：

MILLS, DR ^{[1
]}

KRAMER, FR ^{[1
]}

机构：

[1] COLUMBIA UNIV COLL PHYS & SURG, DEPT HUMAN GENET & DEV, NEW YORK, NY 10032 USA

来源：

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA | 1979年 / 76卷 / 05期

关键词：

D O I：

10.1073/pnas.76.5.2232

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Substitution of inosine for granosine in the nucleic acid fragments synthesized for the sequencing of RNA effectively prevents the formation of secondary structures during electrophoretic analysis. Consequently, the mobility of each fragment in the sequencing gel is a strict function of its molecular weight. Inosine substitution should markedly improve the resolution that can be obtained in the sequencing of DNA as well as RNA.

引用

页码：2232 / 2235

页数：4

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