SEPARATION OF THE ALPHA-CHAINS OF TYPE-I AND TYPE-III COLLAGENS BY SDS-POLYACRYLAMIDE GEL-ELECTROPHORESIS

被引：133

作者：

HAYASHI, T

NAGAI, Y

机构：

[1] Department of Tissue Physiology, Medical Research Institute, Tokyo Medical and Dental University, Chiyoda-ku, Tokyo 101, Kandasurugadai

来源：

JOURNAL OF BIOCHEMISTRY | 1979年 / 86卷 / 02期

关键词：

D O I：

10.1093/oxfordjournals.jbchem.a132544

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A method for the separation of type III collagen from type I collagen by SDS-polyacrylamidegel electrophoresis has been developed. This is based on the observation that the presence of 3-4 M urea decreases the mobility of the α1[III] chain to a greater extent than those of the α1[I] and α2 chains, although the α1[I] and α1[III] chains move at the same rate in the absence of urea. An attempt to separate the α1[II] chain of type II collagen from the α1[I] chain was unsuccessful under the experimental conditions employed. © 1979 by The Journal of Biochemistry.

引用

页码：453 / 459

页数：7

共 15 条

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