MATRIX-ASSISTED LASER DESORPTION IONIZATION MASS-SPECTROMETRY OF NUCLEIC-ACIDS WITH WAVELENGTHS IN THE ULTRAVIOLET AND INFRARED

被引：262

作者：

NORDHOFF, E ^{[1
]}

INGENDOH, A ^{[1
]}

CRAMER, R ^{[1
]}

OVERBERG, A ^{[1
]}

STAHL, B ^{[1
]}

KARAS, M ^{[1
]}

HILLENKAMP, F ^{[1
]}

CRAIN, PF ^{[1
]}

机构：

[1] UNIV UTAH,DEPT MED CHEM,SALT LAKE CITY,UT 84112

来源：

RAPID COMMUNICATIONS IN MASS SPECTROMETRY | 1992年 / 6卷 / 12期

关键词：

D O I：

10.1002/rcm.1290061212

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A number of different matrices have been tested and compared for ultraviolet and infrared (UV and IR) matrix-assisted laser desorption/ionization (MALDI) of oligodeoxyribonucleotides and mixtures thereof, as well as ribonucleic acids (tRNA from yeast and rRNA from E. coli). A new technique for removing alkali cations from nucleic acid samples during sample preparation on the sample support is demonstrated. The amount of oligonucleotide sample consumed during a typical measurement in IR-MALDI-MS was determined.

引用

页码：771 / 776

页数：6

共 14 条

[1]

BORNSEN KO, 1990, CHIMIA, V44, P412

[2]

HILLENKAMP F, 1990, BIOLOGICAL MASS SPECTROMETRY /, P49

[3] ENERGETICS OF GRAMICIDIN-S AFTER UV LASER DESORPTION FROM A FERULIC ACID MATRIX [J].