A FISSION YEAST GENE-MAPPING CLOSE TO SUC1 ENCODES A PROTEIN CONTAINING 2 BROMODOMAINS

被引：3

作者：

AVES, SJ ^{[1
]}

HINDLEY, J ^{[1
]}

PHEAR, GA ^{[1
]}

TONGUE, N ^{[1
]}

机构：

[1] UNIV BRISTOL, DEPT BIOCHEM, BRISTOL BS8 1TD, AVON, ENGLAND

来源：

MOLECULAR AND GENERAL GENETICS | 1995年 / 248卷 / 04期

关键词：

SCHIZOSACCHAROMYCES POMBE; BROMODOMAIN; TRANSCRIPTIONAL ACTIVATION; COACTIVATOR; STATIONARY PHASE;

D O I：

10.1007/BF02191650

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A novel gene, brd1, has been cloned from the fission yeast Schizosaccharomyces pombe. The predicted brd1 product contains two copies of an imperfect repeat of 96 amino acid residues in its N-terminal half. These each include a region with high homology to the bromodomains found in transcriptional activator proteins from a diversity of eukaryotes. An in vivo deletion of the complete brd1 open reading frame is not lethal but cells exhibit thermosensitivity, with reductions in both cell growth and stationary phase survival at 36 degrees C, brd1 maps adjacent to the gene suc1, but is expressed separately to give a low abundance 2.1 kb mRNA.

引用

页码：491 / 498

页数：8

共 43 条

[1] SHOTGUN DNA SEQUENCING USING CLONED DNASE I-GENERATED FRAGMENTS [J].

ANDERSON, S .

NUCLEIC ACIDS RESEARCH, 1981, 9 (13) :3015-3027

[2] HIGH-FREQUENCY TRANSFORMATION OF THE FISSION YEAST SCHIZOSACCHAROMYCES-POMBE [J].

BEACH, D ;

NURSE, P .

NATURE, 1981, 290 (5802) :140-142

[3]

CONAWAY RC, 1993, ANNU REV BIOCHEM, V62, P161

[4] A COMPREHENSIVE SET OF SEQUENCE-ANALYSIS PROGRAMS FOR THE VAX [J].

DEVEREUX, J ;

HAEBERLI, P ;

SMITHIES, O .

NUCLEIC ACIDS RESEARCH, 1984, 12 (01) :387-395

[5]

GANSHEROFF LJ, 1995, GENETICS, V139, P523

[6] 2 DISTINCT YEAST TRANSCRIPTIONAL ACTIVATORS REQUIRE THE FUNCTION OF THE GCN5 PROTEIN TO PROMOTE NORMAL LEVELS OF TRANSCRIPTION [J].

GEORGAKOPOULOS, T ;

THIREOS, G .

EMBO JOURNAL, 1992, 11 (11) :4145-4152

[7] GENETIC-ENGINEERING OF SCHIZOSACCHAROMYCES-POMBE - A SYSTEM FOR GENE DISRUPTION AND REPLACEMENT USING THE URA4 GENE AS A SELECTABLE MARKER [J].

GRIMM, C ;

KOHLI, J ;

MURRAY, J ;

MAUNDRELL, K .

MOLECULAR & GENERAL GENETICS, 1988, 215 (01) :81-86

[8] THE FISSION YEAST-CELL CYCLE CONTROL GENE CDC2 - ISOLATION OF A SEQUENCE SUC1 THAT SUPPRESSES CDC2 MUTANT FUNCTION [J].

HAYLES, J ;

BEACH, D ;

DURKACZ, B ;

NURSE, P .

MOLECULAR & GENERAL GENETICS, 1986, 202 (02) :291-293

[9] SUC1 IS AN ESSENTIAL GENE INVOLVED IN BOTH THE CELL-CYCLE AND GROWTH IN FISSION YEAST [J].

HAYLES, J ;

AVES, S ;

NURSE, P .

EMBO JOURNAL, 1986, 5 (12) :3373-3379

[10] THE DROSOPHILA-FSH LOCUS, A MATERNAL EFFECT HOMEOTIC GENE, ENCODES APPARENT MEMBRANE-PROTEINS [J].

HAYNES, SR ;

MOZER, BA ;

BHATIADEY, N ;

DAWID, IB .

DEVELOPMENTAL BIOLOGY, 1989, 134 (01) :246-257

← 1 2 3 4 5 →