CHEMICAL SYNTHESIS OF GLUTAMIC ACID SPECIFICALLY LABELLED WITH DEUTERIUM OR TRITIUM IN ALPHA OR 2 POSITION

被引：6

作者：

HOCHREITER, MC

SCHELLEN.KA

机构：

[1] Department of Physiological Chemistry, Johns Hopkins University School of Medicine, Baltimore, Maryland

来源：

JOURNAL OF LABELLED COMPOUNDS | 1969年 / 5卷 / 03期

关键词：

D O I：

10.1002/jlcr.2590050310

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

Glutamic acid specifically labelled with isotopic hydrogen in the 2 position was produced by exchange equilibration with labelled protons during the reaction of acetylglutamic acid with acetic acid and acetic anhydride. The position of label was established by n.m.r. and mass spectrometry of the deuterium‐labelled compound, and by transaminase‐catalyzed exchange with protons in the case of tritium‐labelled compound. The specific and complete exchange obtained in our experiments contrasts with the previously reported virtual nonlabelling under somewhat different conditions in which the presumed azlactone enolized and hydrolysed in an aqueous medium. Copyright © 1969 John Wiley & Sons, Ltd.

引用

页码：270 / +

页数：1

共 16 条

[1] THE DEHYDRATION AND RACEMISATION OF N-ACYL-L-ASPARTIC ACIDS BY ACETIC ANHYDRIDE [J].