MEASUREMENT OF DNA LENGTH BY GEL-ELECTROPHORESIS

被引:336
作者
SOUTHERN, EM
机构
关键词
D O I
10.1016/0003-2697(79)90235-5
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
Plotting fragment length against reciprocal of mobility gives a straight line over a wider range than the conventional semilogarithmic plot. Curvature can be removed by a simple correction. A method is also given for determining molecular weights from mobilities by direct calculation. © 1979.
引用
收藏
页码:319 / 323
页数:5
相关论文
共 7 条
[1]   ROLE OF MOLECULAR CONFORMATION IN DETERMINING ELECTROPHORETIC PROPERTIES OF POLYNUCLEOTIDES IN AGAROSE-ACRYLAMIDE COMPOSITE GELS [J].
FISHER, MP ;
DINGMAN, CW .
BIOCHEMISTRY, 1971, 10 (10) :1895-&
[2]   CHAIN-LENGTH DETERMINATION OF SMALL DOUBLE-STRANDED AND SINGLE-STRANDED-DNA MOLECULES BY POLYACRYLAMIDE-GEL ELECTROPHORESIS [J].
MANIATIS, T ;
JEFFREY, A ;
VANDESANDE, H .
BIOCHEMISTRY, 1975, 14 (17) :3787-3794
[3]   NEW METHOD FOR SEQUENCING DNA [J].
MAXAM, AM ;
GILBERT, W .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1977, 74 (02) :560-564
[4]   ANALYSIS OF RESTRICTION FRAGMENTS OF T7 DNA AND DETERMINATION OF MOLECULAR-WEIGHTS BY ELECTROPHORESIS IN NEUTRAL AND ALKALINE GELS [J].
MCDONELL, MW ;
SIMON, MN ;
STUDIER, FW .
JOURNAL OF MOLECULAR BIOLOGY, 1977, 110 (01) :119-146
[5]   CLONING OF YEAST RIBOSOMAL DNA REPEAT UNIT IN SSTI AND HINDIII LAMBDA VECTORS USING GENETIC AND PHYSICAL SIZE SELECTIONS [J].
PHILIPPSEN, P ;
KRAMER, RA ;
DAVIS, RW .
JOURNAL OF MOLECULAR BIOLOGY, 1978, 123 (03) :371-386
[6]   PREPARATIVE GEL-ELECTROPHORESIS APPARATUS FOR LARGE-SCALE SEPARATIONS [J].
SOUTHERN, EM .
ANALYTICAL BIOCHEMISTRY, 1979, 100 (02) :304-318
[7]   NUCLEOTIDE-SEQUENCE OF SV40 DNA RESTRICTION FRAGMENT HIND C-HAP2 [J].
VANHEUVERSWYN, H ;
VANDEVOORDE, A ;
FIERS, W .
NUCLEIC ACIDS RESEARCH, 1977, 4 (04) :1015-1024