DIRECT SEQUENCING OF DOUBLE-STRANDED POLYMERASE CHAIN REACTION-AMPLIFIED 16S RDNA

被引：21

作者：

BOTH, B

KRUPP, G ^{[1
]}

STACKEBRANDT, E

机构：

[1] UNIV KIEL, INST GEN MICROBIOL, BOT GARTEN 9, W-2300 KIEL 1, GERMANY

[2] UNIV QUEENSLAND, DEPT MICROBIOL, ST LUCIA, QLD 4072, AUSTRALIA

来源：

ANALYTICAL BIOCHEMISTRY | 1991年 / 199卷 / 02期

关键词：

D O I：

10.1016/0003-2697(91)90092-8

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A number of different procedures have been developed for direct sequence analysis of PCR products. These methods rely on the cumbersome isolation of specific PCR products from agarose gels or the production of single-stranded template DNAs. In the approach presented here, we describe primers for the amplification of 16-S rDNA and a simple preparation of PCR product for sequencing. © 1991.

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页码：216 / 218

页数：3

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