PREPARATION OF 14,38-BIS-[S-CARBAMIDOMETHYL]-(BASIC TRYPSIN INHIBITOR) POSSESSING FULL BIOLOGICAL ACTIVITY

被引：44

作者：

LIU, WK

MEIENHOFER, J

机构：

[1] Children's Cancer Research Foundation, The Children's Hospital Medical Center Boston

[2] Harvard Medical School, Boston

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1968年 / 31卷 / 03期

关键词：

D O I：

10.1016/0006-291X(68)90500-7

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Dithiothreitol reduced selectively the disulfide bond linking the half cystine residues 14 and 38 of bovine basic trypsin inhibitor in the absence of urea at pH 8.2. Iodoacetamide treatment afforded 14, 38-bis-[S-carbamidomethyl]-(basic trypsin inhibitor) possessing full biological activity. © 1968.

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页码：467 / +

页数：1

相关论文

共 18 条

[1]

AUHAGEN E, 1967, DRUGS ANIMAL ORIGIN, P69

[2] HUMAN PITUITARY GROWTH HORMONE .16. REDUCTION WITH DITHIOTHREITOL IN ABSENCE OF UREA [J].

BEWLEY, TA ;

DIXON, JS ;

LI, CH .

BIOCHIMICA ET BIOPHYSICA ACTA, 1968, 154 (02) :420-&

[3] STRUCTURE PRIMAIRE DUN INHIBITEUR PANCREATIQUE DE LA TRYPSINE (INHIBITEUR DE KUNITZ ET NORTHROP) .2. CARACTERISATION DES PEPTIDES RESULTANT DE LHYDROLYSE TRYPSIQUE [J].

CHAUVET, J ;

NOUVEL, G ;

ACHER, R .

BIOCHIMICA ET BIOPHYSICA ACTA, 1966, 115 (01) :130-&

[4]

CHAUVET J, 1967, J BIOL CHEM, V242, P4274

[5] DITHIOTHREITOL NEW PROTECTIVE REAGENT FOR SH GROUPS [J].

CLELAND, WW .

BIOCHEMISTRY, 1964, 3 (04) :480-&

[6] RETENTION OF BIOLOGICAL POTENCY OF HUMAN PITUITARY GROWTH HORMONE AFTER REDUCTION AND CARBAMIDOMETHYLATION [J].

DIXON, JS ;

LI, CH .

SCIENCE, 1966, 154 (3750) :785-&

[7] PREPARATION AND PROPERTIES OF 2 NEW CHROMOGENIC SUBSTRATES OF TRYPSIN [J].

ERLANGER, BF ;

COHEN, W ;

KOKOWSKY, N .

ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS, 1961, 95 (02) :271-&

[8] A MODIFIED SPECTROPHOTOMETRIC DETERMINATION OF CHYMOTRYPSIN, TRYPSIN, AND THROMBIN [J].

HUMMEL, BCW .

CANADIAN JOURNAL OF BIOCHEMISTRY AND PHYSIOLOGY, 1959, 37 (12) :1393-1399

[9] BASIC TRYPSIN INHIBITOR OF BOVINE PANCREAS .V. DISULFIDE LINKAGES [J].

KASSELL, B ;

LASKOWSK.M .

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1965, 20 (04) :463-&

[10]

KATZ AM, 1959, J BIOL CHEM, V234, P2897