EVIDENCE FOR THE INVOLVEMENT OF VICINAL SULFHYDRYL-GROUPS IN THE INSULIN STIMULATION OF INTRACELLULAR GLUCOSE-METABOLISM IN ZAJDELA HEPATOMA-CELLS

被引：10

作者：

CARON, M ^{[1
]}

CHERQUI, G ^{[1
]}

WICEK, D ^{[1
]}

MELIN, B ^{[1
]}

REYNET, C ^{[1
]}

CAPEAU, J ^{[1
]}

PICARD, J ^{[1
]}

机构：

[1] UNIV PARIS 06,INSERM,U181,BIOCHIM LAB,27 RUE CHALIGNY,F-75571 PARIS 12,FRANCE

来源：

BIOSCIENCE REPORTS | 1990年 / 10卷 / 01期

关键词：

glucose metabolism; insulin; phenylarsine oxide; Zajdela hepatoma cells;

D O I：

10.1007/BF01116847

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Phenylarsine oxide (PhAsO), a dithiol reagent that blocks insulin stimulation of glucose transport in 3T3 L1 cells, also altered insulin stimulation of intracellular glucose metabolism in Zajdela Hepatoma cultured cells. PhAsO (2 μM) similarly inhibited the insulin-induced glycogen and lipid syntheses without modifying the basal level of these processes, cell viability or the ATP content. Prior incubation of the cells with PhAsO did not prevent insulin binding to the cells, or activation of the receptor tyrosine kinase, while it minimally (16%) altered receptor internalization. These results indicate that cellular dithiols located at a post-receptor step are involved in the transduction of the insulin signal to intracellular glucose metabolism. © 1990 Plenum Publishing Corporation.

引用

页码：23 / 29

页数：7

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