AN SRY-RELATED GENE EXPRESSED DURING SPERMATOGENESIS IN THE MOUSE ENCODES A SEQUENCE-SPECIFIC DNA-BINDING PROTEIN

被引：242

作者：

DENNY, P ^{[1
]}

SWIFT, S ^{[1
]}

CONNOR, F ^{[1
]}

ASHWORTH, A ^{[1
]}

机构：

[1] INST CANC RES,CHESTER BEATTY LABS,FULHAM RD,LONDON SW3 6JB,ENGLAND

来源：

EMBO JOURNAL | 1992年 / 11卷 / 10期

基金：

英国惠康基金;

关键词：

HMG-BOX; SEX DETERMINATION; TRANSCRIPTION FACTOR;

D O I：

10.1002/j.1460-2075.1992.tb05455.x

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

SRY, the testis determining gene, encodes a member of a family of DNA binding proteins characterized by an amino acid sequence motif known as the HMG box. Using degenerate primers and the polymerase chain reaction, we have isolated SRY-related cDNAs from adult murine testis RNA. One of these, Sox-5, encodes a 43 kDa HMG-box protein with similarities to transcription activating proteins. Anti-Sox-5 antibody was used to analyse expression of Sox-5 in pre-pubertal testis and in fractionated spermatogenic cells. Sox-5 is restricted to post-meiotic germ cells, being found at highest levels in round spermatids. Sox-5 is a DNA binding protein and binding site selection assays suggest that it can bind specifically to oligonucleotides containing the consensus motif AACAAT. Sry can also bind to this motif, indicating that the Sry, family may have overlapping sequence specificities.

引用

页码：3705 / 3712

页数：8

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