CELL-ADHESION ACTIVITY OF A 30-KDA MAJOR SECRETED PROTEIN FROM HUMAN BLADDER-CARCINOMA CELLS

被引：57

作者：

AKAOGI, K

OKABE, Y

FUNAHASHI, K

YOSHITAKE, Y

NISHIKAWA, K

YASUMITSU, H

UMEDA, M

MIYAZAKI, K

机构：

[1] YOKOHAMA CITY UNIV,KIHARA INST BIOL RES,DIV CELL BIOL,MINAMI KU,YOKOHAMA 232,JAPAN

[2] KANAZAWA MED UNIV,DEPT BIOCHEM,UCHINADA,ISHIKAWA 92002,JAPAN

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1994年 / 198卷 / 03期

关键词：

D O I：

10.1006/bbrc.1994.1149

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Human bladder carcinoma cell line EJ-1 secreted a protein of 30 kDa as a major component. This protein was purified from the serum-free conditioned medium of EJ-1. Its N-terminal amino acid sequence was identical to that of fibroblast-derived endothelial cell growth factor (f-ECGF), but there were differences in their amino acid compositions and isoelectric points. The 30-kDa protein showed no effect on the growth of bovine caspillary endothelial cells, but it promoted the attachment and spreading of rat liver cell line BRL and human umbilical vein endothelial cell line ECV-304. These results suggest that the 30-kDa protein, tentatively named “tumor-derived adhesion factor (TAF)”, may be related with the aberrant cell adhesion of cancer cells. © 1994 Academic Press, Inc.

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页码：1046 / 1053

页数：8

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