INTERACTION OF TOCOPHEROL WITH PEROXYL RADICALS DOES NOT LEAD TO THE FORMATION OF LIPID HYDROPEROXIDES IN LIPOSOMES

被引:12
作者
DMITRIEV, LF
IVANOVA, MV
LANKIN, VZ
机构
[1] Group of Lipid Biochemistry, Cardiology Research Centre, 121552 Moscow
关键词
TOCOPHEROL; SUPEROXIDE ANION; FREE RADICAL SCAVENGING; LIPID PEROXIDATION; REDOX REACTIONS;
D O I
10.1016/0009-3084(94)90025-6
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
alpha-Tocopherol (alpha-TOH) is thought to act as a lipid-soluble antioxidant by scavenging the chain-carrying lipid peroxyl radical (LOO(.)), giving rise to alpha-TO. and LOOH. The aim of the present work is to test the possibility that alpha-TOH may also exert a protective action by converting LOO(.) into the parent, unoxidized lipid molecule (LH) with concomitant formation of O-2(-) and T+O. To assess the relative contribution of the two pathways, the amounts of products accumulating were measured, i.e. LOOH and O-2(-), under conditions where lipid peroxidation was initiated at linear rates by the UV light-induced decomposition of AIBN. The overall conclusion of the study is based on the observation that significant amounts of O-2(-) were produced, whereas the Delta LOOH/Delta alpha-TOH was considerably smaller than 1.0. A mechanism of one or two-electron oxidation of TOH is considered; according to it the phospholipid primary structure is restored (LOO(.) --> LH, breaking of the C-O bond). This leads to the oxygen or superoxide anion-radical release accordingly.
引用
收藏
页码:35 / 39
页数:5
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