RESOLUTION OF CIRCULAR, NICKED CIRCULAR AND LINEAR DNA, 4.4 KB IN LENGTH, BY ELECTROPHORESIS IN POLYACRYLAMIDE SOLUTIONS

被引：19

作者：

GARNER, MM

CHRAMBACH, A

机构：

[1] Section Macromolecular Analysis, Laboratory of Theoretical and Physical Biology, National Institute of child Health and Human Development, National Institutes of Health, Bethesda, Maryland

来源：

ELECTROPHORESIS | 1992年 / 13卷 / 03期

关键词：

D O I：

10.1002/elps.1150130136

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Circular DNA of more than 1,400 bp in size is known not to migrate into polyacrylamide gels. The migration of supercoiled plasmid pBR322 DNA (4,363 bp) into uncrosslinked polyacrylamide (M(w) 5 X 10(6)) solutions and its separation, on the basis of conformation, from its nicked form is demonstrated in this study. Migration of the supercoiled, nicked circular and linear forms of the plasmid DNA is retarded in proportion to the concentration of uncrosslinked polyacrylamide, the degree of retardation being highest for the nicked circular form. Decreasing the level of supercoiling of the covalently closed circular form by decreasing the concentration of the intercalating dye (ethidium homodimer) shows that the degree of retardation decreases in proportion to the superhelix density.

引用

页码：176 / 178

页数：3

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