METABOTROPIC RESPONSES TO ACETYLCHOLINE AND SEROTONIN OF XENOPUS OOCYTES INJECTED WITH RAT-BRAIN MESSENGER-RNA ARE TRANSDUCED BY DIFFERENT G-PROTEIN SUBTYPES

被引：23

作者：

KANEKO, S ^{[1
]}

TAKAHASHI, H ^{[1
]}

SATOH, M ^{[1
]}

机构：

[1] KYOTO UNIV,FAC PHARMACEUT SCI,DEPT PHARMACOL,KYOTO 60601,JAPAN

来源：

FEBS LETTERS | 1992年 / 299卷 / 02期

关键词：

GTP-BINDING PROTEIN; MUSCARINIC RECEPTOR; 5-HT1C RECEPTOR; PHOSPHOLIPASE-C; ANTISENSE DNA; XENOPUS OOCYTE;

D O I：

10.1016/0014-5793(92)80242-9

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

To assign the GTP-binding protein (G-protein) subtype involved in the signal transduction from exogenous receptors to phospholipase C in the Xenopus oocyte translation system, antisense DNA complementary to rat G-protein alpha-subunit mRNA was designed and injected together with rat brain poly(A)+ RNA. Current response of mRNA-injected oocytes to acetylcholine (ACh) was suppressed dose-dependently by a co-injection of G(i1)alpha-antisense DNA, but response of the same oocytes to serotonin (5-HT) was not inhibited. In the oocytes co-injected with G(o)alpha-antisense DNA, the 5-HT response was more effectively suppressed than the ACh response. These results suggest that G(o)alpha but not G(i1)alpha intermediates brain 5-HT1C receptor function, and in contrast, muscarinic receptors derived from rat brain utilize G(i1)alpha rather than G(o)alpha to activate phospholipase C.

引用

页码：179 / 182

页数：4

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