STEROID-PROTEIN INTERACTIONS .22. EXTRAPOLATION TECHNIQUE FOR DETERMINATION OF CORTICOSTEROID-BINDING GLOBULIN BY GEL FILTRATION

被引：25

作者：

HOFFMANN, W

WESTPHAL, U

机构：

[1] Biochemistry Department, University of Louisville School of Medicine, Louisville, KY 40208, Reynolds Building

来源：

ANALYTICAL BIOCHEMISTRY | 1969年 / 32卷 / 01期

关键词：

D O I：

10.1016/0003-2697(69)90103-1

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A method has been developed for the quantification of the corticosteroid-binding globulin (CBG) which eliminates the disadvantages of the gel filtration technique (dissociation, possible reversible adsorption). Binding capacity values are obtained with three different amounts of Sephadex G-50, and are extrapolated to a gel volume of zero. CBG concentrations have been measured for two normal and two pregnancy sera. The results have been found in agreement with those obtained by two independent procedures based on equilibrium dialysis. © 1969.

引用

页码：48 / &

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